Role of CRISPR in Crop Improvement: A Review
Hassan Raza *
Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Muhammad Ashar Abdullah
Center of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan.
Samar Khaliq
Department of Botany, University of Agriculture Faisalabad, Pakistan.
Muhammad Haider Sajjad
Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan.
Mehboob Elahi
Department of Agronomy, University of Agriculture Faisalabad, Pakistan.
Muhammad Raffay Khan
Department of Agronomy, University of Agriculture Faisalabad, Pakistan.
Abdullah Siddique
Department of Agronomy, University of Agriculture Faisalabad, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
The meals and agriculture sectors have witnessed good sized advancements due to the state-of-the-art improvements in agricultural biotechnology and genetic engineering, that have more advantageous the essential characteristics of plant agronomic tendencies. A extensively used technique for inducing focused deletions, insertions, and precise sequence adjustments throughout numerous species and mobile kinds is collection-particular nucleases (SSNs)-based centered genome editing. Commercial adoption of genome modifying gear, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and siRNA-mediated RNA interference, has been significant. However, the whole landscape of lifestyles sciences underwent a paradigm shift with the invention of the CRISPR/Cas9 machine as a flexible tool for genome modifying. Initially recognized as a virological defense DNA segment in bacteria and archaea, the clustered Regularly interspaced short palindromic repeats (CRISPR) machine has revolutionized molecular biology. Through modern molecular organic strategies, CRISPR/Cas9 enables unique modifications in any crop species. Its efficacy, reproducibility, and specificity have earned CRISPR/Cas9 the moniker of a "leap forward" in biotechnology.
Keywords: Genome editing, CRISPR/Cas, crop improvement, gene function, palindromic, commercial adoption, crop species